SIM agar deep and observed the reaction. Each test performed identified was used to determine the specifics and identify the unknown bacterium. The Methyl Red test produced an orange color, which was a negative result. No bubble would be a negative result. The VP part of the MR-VP test produced an orange color as well, which is a negative result.
Incubated for 24Hrs, recovered, and checked for optimal temperature for growth of unknown; which was the LB agar at 37 degrees. We will write a custom essay sample. The unknown culture was inoculated in the Tryptic Soy Broth tube, a TSA plate agar, and a TSA slant.
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The media in this tube contained the indicator Phenol Red. The slide was placed back on the staining rack. The SIM media was cloudy around the area where I inoculated the bacteria, indicating it is positive for motility. Identifying the unknown bacterium was determined by separating and differentiating possible bacteria based on specific biochemical characteristics. All of the following tests were performed on this unknown on February 09, 2008. After one minute, the slide was rinsed one final time on the small sink followed by blotting the stained slide with Bibulous paper. Each student chose their own unknown bacteria according to the number. Procedures were followed as stated in the course laboratory manual provided by the instructor, unless otherwise noted. Next, the biochemical reactions tests are performed. I let them incubate until the next class period.